Monthly Archives: May 2012

Thursday 05/10/12

Posted on May 10, 2012 by admin

10:00A -8:00P working on chromatic correction for beads to apply to molecule lists Finished script to merge multiple bead fields from confocal and compute transforms. working on intro sections of ESF talk, formatted to work on laptop. comments back from … Continue reading →

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Wednesday 05/09/12

Posted on May 9, 2012 by admin

12:30 P – 9:45 (volleyball practice 8:30A-11:30A) Pellet and resuspend all new probes. updated probe & plasmid database with new & resynthesized probes: [spreadsheet 0AjSqkxgziU1YdHlnZmFzOVBsX0JQUjlrQnRUWjVYVGc 600 400 sheet=1] 3x maternal Gal4 flies arrived from Perrimon lab -> start expanding coding molecule … Continue reading →

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Tuesday 05/08/12

Posted on May 8, 2012 by admin

10:00A – 10:50 P Bcd Gal4 x shRNA Pc2 and x Psc shRNA1 both still hatch larvae. Flip collection cages check mail for Perrimon Gal4 3x line – not yet arrived rerunning Pc-GFP controls with Colenso’s parameters (min height 750 … Continue reading →

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Monday 05/07/12

Posted on May 7, 2012 by admin

9:50A – 8:30 P Sang-Hee lab meeting Sonny JC (Ringrose paper and Psc as cell cycle regulator paper) laying out presentation for ESF conference

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Saturday 05/05/12

Posted on May 5, 2012 by admin

11:00A -5:30P Talk title for Poland wash out primary antibodies Nanodrop gel-extraction samples STORM cryo-section samples: considerable out of focus light makes for hazy looking conventional images, though nuclei and membranes are clearly distinguishable in the respective channels. 647 STORM … Continue reading →

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Friday 05/04/12

Posted on May 4, 2012 by admin

12:00 P – 9:30 P 8:30-12P volleyball practice update probe & plasmid library update antibody labels collect virgins set up shRNA crosses x Pc shRNA2 and Psc shRNA 1 in collection cages PCR for in vitro transcription rxn to make … Continue reading →

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Thursday 05/03/12

Posted on May 3, 2012 by admin

8:30 A – 8:45P, 10:00P – 11:30P cryo-sectioning: cut 7 slides — embryos mostly around edge.  Need larger cutting surface for good sections. finish STORM run, shoot beads collect virgin Gal4-Bcd3’UTR in situs day 3 fixed issue in list2tif_color_drift_correct: wasn’t … Continue reading →

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Wednesday 05/02/12

Posted on May 2, 2012 by admin

10:25 A – 8:50P Wash out O/N H3K27me3 on Pc-GFP labeling embryos Etch H4 (H3K27, K4): 1h 25 min sequences back from GeneWiz: Fab8B +/-, inside msa ncRNA (-) Fab8A, +/- inside msa ncRNA (-) AbdB-2B +/+ antisense. use T7 … Continue reading →

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Tuesday 05/01/12

Posted on May 1, 2012 by admin

11:00 A – 6:45P, 8:00P -12:00A flip fly collection cages, 11A Miniprep cultured colonies: 8E, 7E, 2E, AbdB2 and Fab8 (2 colonies each).  Send for sequencing. Rearrange ultratome room, suspend on air-table, clean room. Flip collection cages, 3:15 A Analyzing … Continue reading →

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