Monthly Archives: July 2012

issues with computing photon counts by exponential distributions

Posted on July 9, 2012 by admin

Fitting exponential distributions to molecule areas output from insight, multiplied by photon per count for gain 30  on STORM2 (.41 photons).       These data are all from Hazen’s 2D microtubules.  My 3D microtubules, Yari’s 2D sections, my 2D … Continue reading →

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07/08/12

Posted on July 8, 2012 by admin

3:30 P – 6:10 P Trimming H3K4me3 4-color, H3, and Dm01 4-color labeled samples.

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07/07/12

Posted on July 7, 2012 by admin

3:30 P – 6:30P Troubleshooting STORM analysis code. Comparing photon counts from Yari and Hazen’s data to my data.  Yari ~ equal my data (2% more photons in 647, 5,200 uncorrected).  Hazen’s data has 7,000+ uncorrected (no Notch filters or … Continue reading →

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Friday 07/06/12

Posted on July 6, 2012 by admin

10:10 A – 7:30P, 9:00P -11:15P Flip fly cages, 10:10A Revisions of dynamics paper, revised, updated to v7. compare to NM requirements.  Version and comparison sent back to ML.  May need to combine figures and prune text. Take embryos out … Continue reading →

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Thursday 07/5/12

Posted on July 5, 2012 by admin

9:50A – 7:15P, 9:00P – 10:45P check nascent transcripts of AbdA (red) and Antp (green) on yw and Pc O/N short-fix embryos.  No obvious staining on turnkey. Should check 6/11 embryos as positive controls and 6/25 embryos to see effect … Continue reading →

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Probe tests, June 2012

Posted on July 5, 2012 by admin
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last two weeks in images

Posted on July 5, 2012 by admin
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Wednesday 07/04/12

Posted on July 4, 2012 by admin

12:30 P – 6:45P, 7:45P – 10:10P wash out primary antibodies. dispatched old reserves of dilute, reused primary antibodies.  Moved new batch to freezer.  Will test stain later — should preserve better for one extra round. Test DNA-FISH probes.  centromeric … Continue reading →

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Tuesday 07/03/12

Posted on July 3, 2012 by admin

10:00 A – 7:10P, 8:45P-11:00P Sent version 4 of snail dynamics manuscript to ML Flip collection cages. 12:30 PM fix Pc-GFP and yw embryos.  15 min fix.  Rehydrating embryos to test protein stains. Image sections on Turnkey.  sections substantially non-flat. … Continue reading →

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Monday 07/02/12

Posted on July 2, 2012 by admin

10:15 A – 6:30P, 8:30-9:30P Flip collection cages, 10:15 A Finishing embedding of SuZ labeled embryos HCBI access expired. Need to fill out paperwork again to restore access. Analyzing previous Pc-GFP images.  z-calibration not as good as recent attempts with … Continue reading →

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