Monthly Archives: November 2012

Thursday 11/23/12

Posted on November 21, 2012 by admin

10:30 A – 7:30 P Thanksgiving Fly work expand YWs Flip stocks check crosses.  double HbZ not yet emerging.  recombineering crosses looking bleak, we’ll see in anotehr 5-6 days… Probe making New library stats spin down dissolving probe.  Gently Pippett … Continue reading →

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Tuesday 11/20/12

Posted on November 20, 2012 by admin

9:45 A – 11:30 P Confocal Test protein stain (didn’t look good in conventional images) H3K27me3 weakly detectable. H3K9me2 not stained. Psc, SuZ(12), not well stained. compared to DFISH S2 cell (slide #1 in record, 10/23)  All channels (except 1:100 … Continue reading →

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Monday 11/19/12

Posted on November 19, 2012 by admin

9:15 A — 7:15 P, 8:15P – 1:15 A Cell labeling hot washes for probe first slide (2x at 60C) 1x at RT remove and save primary antibodies at RT for primary first slide. PBT rinses (3x soln change, 3rd … Continue reading →

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Protected: Notes – Lab meeting 11/19/12 Graham

Posted on November 19, 2012 by admin

There is no excerpt because this is a protected post.

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STORM2 rebuild SOP

Posted on November 19, 2012 by admin

  Follow text file SOP for 750 laser. Aligning back optics place microscope in epi mode (12.000) Course tuning remove 20x, 100x objectives. screw in target objective in 100x objective position. remove final lens before scope body, add iris in … Continue reading →

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Sunday 11/18/12

Posted on November 18, 2012 by admin

10:15 A – 6:50 P, 7:50P – 10:30P Probe making have not confirmed nicking enzyme concentration for digestion.  Canceled PAGE introduction with Bryan, will attempt again later. Telomeres Read over telomere manuscript from John Wu  Imaging telomeres: 656 = 60 … Continue reading →

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Saturday 11/17/12

Posted on November 17, 2012 by admin

10:00 A – 12:00P, 3:30P – 8:00P, 9:00P-11:20P S2 Cell cent + chromatin/PcG Hot washes PBT washes block 4 hrs antibodies: H3K9me2, Pc, PCNA (Fisher) (1:3 across the top) rinse, block, secondaries O/N at 4C Harvard wins Yale Game Chromatic … Continue reading →

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Friday 11/16/12

Posted on November 16, 2012 by admin

9:45 A – 7:00P, 8:00P – 10:30P Making summary images of SCM data. Reconcentrating / Precipitating probe prior to nicking reaction. Combined into 2 tubes. Dissolving pellets at 37 O/N.  (waiting for Nicking enzyme to continue). Nicking enzyme arrived Brian … Continue reading →

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Protected: SCM summary

Posted on November 16, 2012 by admin

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Thursday 11/15/12

Posted on November 15, 2012 by admin

9:15 A — 6:45 P, 8:30P – 12:00A collect virgins for sim Espl[D] recombineering new cross, sna males x Sp/CyoZ; TM2/TM6 virgins clear MTDs and TM2/TM6s for virgin collection (peak emergence for latest bottles?) pulverize and refreeze Espl[D] and sim[D] … Continue reading →

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