Monthly Archives: January 2013

Thursday 01/24/13

Posted on January 24, 2013 by admin

9:30 A – 7:30P STORM * Finish O/N run by 10A. * Run Z-calibration * Copy and delete data!  Remove external hard-drive * Image BX-C + H3K27me3-488 slide (at 4C in PBT).  maybe we should store these in 2x SSCT? … Continue reading →

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Wednesday 01/23/13

Posted on January 23, 2013 by admin

10:30 A – 8:30P, 9:30P – 11:45P (8:45-10:30A workout) Fly Work collect virgins: Df/CyO,esc[2],  2x mat-tub Gal4. collect males: esc[6]/Sp. cross (1 new bottle) esc[6]/Sp x Df/CyO,esc[2] (should have used Gla, will need to screen Sp for all flies that … Continue reading →

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DNA FISH on coverglass

Posted on January 23, 2013 by admin

Adopted from Wu lab protocol.  01/23/13 Briefly wash cells with 1x PBS (not PBT) Fix cells with 4% formaldehyde for 15 min Quick wash with 1x PBS Wash cells with 2x SSCT for 5 min Wash cells with 50% formamide/2x … Continue reading →

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STORM2 BX-C 01/23/13

Posted on January 23, 2013 by admin

Setup Back-optics aligned Focus lock working Laser power: 647 laser at 200 mW source: 130 mW at power meter 405 laser: max power = 1.89 mW.  10% = .09 mW z-stage drift without focus lock is ~800 nm per min … Continue reading →

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Tuesday 01/22/12

Posted on January 22, 2013 by admin

  Fly Work collect virgins (df/CyO-Esc[2])  AM # New crosses with remaining MTDs! # collect virgins PM Ultra-cryo sectioning yw embryos Cutting sample at -51C, knife at -54C, TG -50C at .65 – .75 um cuts pretty well, still better … Continue reading →

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Monday 01/21/13

Posted on January 21, 2013 by admin

10:00 – 8:30P, 9:30P-11:00P (9A-10A workout session) 10A-1:30P lab meeting George. Notes Fly work MTD crosses Psc-1 parental cross started at 29C gives viable F1s. Psc-2 no F1 progeny — (need to redo with fresh food + more flies) Rad21 … Continue reading →

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Sunday 01/20/13

Posted on January 20, 2013 by admin

11:00 A – 4:00P, 10:00P-12:00A Coding Frame-by-frame analysis now runs in a platform independent way form the new shared project matlab-storm folder for both DaoSTORM and InsightM Writing new Analysis script (RunDotFinder) — modified, more flexible version of Run_Insight that … Continue reading →

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Denaturing PAGE

Posted on January 20, 2013 by admin

(Adapted from Bryan Harada’s protocol, 02/25/12). for Extraction of single stranded DNA probes Materials: 2x Loading Buffer (96% formamide/20mM EDTA, prepare using freshly deionized formamide) Acrylamide/Bis 19:1 40% (w/v) Solution   TEMED 10% APS (w/v). make fresh from powder and … Continue reading →

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Saturday 01/19/13

Posted on January 19, 2013 by admin

11:00 A – 7:00 P Snail Rewrite fxn_Figs_y_v_snail to render only figures indicated in list Plotnames and show them in the indicated figure: ff = strfind(Plotnames,’box_CoVratio’); ff = find(1 – cellfun(@isempty, ff)); if ~isempty(ff) figure(Plotnumbers(ff)); clf; … end This facilitates … Continue reading →

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Code meeting

Posted on January 19, 2013 by admin

Organization Functions ‘Clean code’ * has help file — lists all inputs and outputs with descriptors functions have small number of mandatory inputs (typically 1-4) additional inputs are passed as ‘Flag’,value pairs.  (see examples) long code is written in demarcated code blocks … Continue reading →

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