Monthly Archives: January 2013

Sunday 01/13/13

Posted on January 13, 2013 by admin

3:30P – 11:55 P Fly work Flipped most of rescue crosses.   Several vials extinct.  Should still have more vials than I want to screen though. New crosses of SCM males to MTD virgins (2 vials). Examine current crosses of … Continue reading →

Posted in Summaries | Tagged , , | Comments Off on Sunday 01/13/13

Saturday 01/12/13

Posted on January 12, 2013 by admin

10:30 A – 8:00P Probe making precipitate probe, resuspend in fresh DNAse free ddH2O snail data analysis run nuc mapping cyto2nucmap for MP08Hz_snaD_2 data modify lsm_viewer to not append embryo numbers when running export all on embryos data without position … Continue reading →

Posted in Chromatin, probe and plasmid building, Summaries | Tagged | Comments Off on Saturday 01/12/13

STORM2 session 01/11/13

Posted on January 12, 2013 by admin

Laser powers 750 at 1700 mA is 209 mW at meter 647 at 200 mW source is 120 at meter.  300 mW source is 190 at meter 488 at 75% power is 200 mW at meter 405 at max is … Continue reading →

Posted in General STORM | Comments Off on STORM2 session 01/11/13

Friday 01/10/13

Posted on January 11, 2013 by admin

8:30 A – 9:50 P Fly work Flip collection plate 8:30A Fixing embryos 11:30A.  (20 min 8% FA reaction) Flip all polycomb mutants into bottles STORM made new bead slide, 1:25K imaging 2 rounds of beads for 3D fitting K9me2 … Continue reading →

Posted in probe and plasmid building, Summaries | Tagged , , | Comments Off on Friday 01/10/13

Thursday 01/10/13

Posted on January 10, 2013 by admin

8:30A – 10:30P Flip collection plate 8:30A Fixing embryos 10:30A.  (20 min 8% FA reaction) O/N confocal runs — 700 at speed 4 still running.  5 not quite as clean. copy data. Make new fly plates (~4 tubes of plates). … Continue reading →

Posted in Summaries | Tagged , , | Comments Off on Thursday 01/10/13

Wednesday 01/09/13

Posted on January 9, 2013 by admin

10:00A — 10:45P Cell culture blocking IF first.   secondaries for IF first (2 m-750 (6:1), 6 wells m-488 (2.0)) 10 min in .1% GA, 4% PFA post fix.  wash in PBT, then 2xSSCT following Brian’s protocol.  Need rubber cement. … Continue reading →

Posted in Summaries | Tagged , , | Comments Off on Wednesday 01/09/13

Tuesday 01/08/13

Posted on January 8, 2013 by admin

10:00A – 10:30P (8:30-10A lab exercise/training) Bistability First draft of deterministic model figure and corresponding text Trying to figure out correct integral to compute an energy surface from the vector field.  Not working so far. Fly work collect virgins: (now … Continue reading →

Posted in Summaries | Tagged , , | Comments Off on Tuesday 01/08/13

Monday 01/07/13

Posted on January 7, 2013 by admin

9:30 A – 8:30P, Probe making Emulsion PCR of engrailed region probe at Wu lab.   Final library concentration 16 ng/uL, in 14 uL.  Should PCR amplify once. Brian will run test PCR and test gel to check library quality … Continue reading →

Posted in Summaries | Tagged , , | Comments Off on Monday 01/07/13

Sunday 01/06/12

Posted on January 6, 2013 by admin

11:00 A – 10:30 P snail analysis Finish O/N confocal of MP06Hz Finish O/N confocal of MP08Hz sna[D] extract images from lsm, analyze nuclear density and record staging run dot-finder to count mRNA MP08Hz conclusions:  2 def. mutants in mid … Continue reading →

Posted in Summaries | Tagged , | Comments Off on Sunday 01/06/12

Saturday 01/05/12

Posted on January 5, 2013 by admin

11:00A – 3:30P, 4:30P – 10:45 P collect virgins DB Sp/CyoZ; TM2/TM6 MTD sna[18]/CyoZ/+; TM3/+/Pr esc[6]/CyO Df/CyO,esc[2] – not needed (need to cross esc[6] to Sp/CyO first). E(spl)[D] sim[D] collect Tub,Hu non-ebony male potential recombinants from 20+ lines.  Cross to … Continue reading →

Posted in Fly Work, Summaries | Comments Off on Saturday 01/05/12