Monthly Archives: February 2013

chromatic warping: general formulation

Posted on February 11, 2013 by admin

Description of problem There are N different color channels which are imaged through different filters, and need to be mapped together to an accuracy greater than the resolution limit of the instrumentation / detection system (e.g. 10-20 nm for STORM, … Continue reading →

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Protected: lab meeting 02/11/13

Posted on February 11, 2013 by admin

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Sunday 02/10/13

Posted on February 10, 2013 by admin

10:30 A – 11:35 P STORM Finish O/N STORM run of Fab7 running new chromatic 3D bead calibration (more positions, slightly narrower z-range) check out Hazen’s new Zee-calibrator — (looks like in progress viewer for data-fit curves). Doesn’t show molecules … Continue reading →

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Friday 02/08/13

Posted on February 9, 2013 by admin

11:00 A – 8:30P, 9:30P-11:00P (remote) Fly work collect virgins non-Sp CyO males of Esc not obviously extra sex combs (only 1 male so far). Need to check bristly number and frequency expected (see Vivek’s paper). new bottles of MTDs … Continue reading →

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Saturday 02/09/13

Posted on February 9, 2013 by admin

11:00 A – 8:30P 9:30 – 10:15P (remote) (blizzard) STORM Finish O/N run of BXC+Pc take new bead calibration movies setup imaging of FAb7+Pc current beadslide has substantial tilt — maybe we replace it sometime Fly work collect virgin mat … Continue reading →

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z-calibration part 4

Posted on February 7, 2013 by admin

There are two problems with the maximal alignment approach: it is still a bit slow (though much faster than the iterative method). it decouples wx and wy, and the simulated annealing allows for some generally small but arbitrary jiggle in … Continue reading →

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Thursday 02/07/13

Posted on February 7, 2013 by admin

9:30 AM – 8:30P STORM Finish O/N STORM imaging Pc-488 not bleaching well. Maybe 750 will be better. Fly Work Esc virgins emerging — collecting. some non-Sp (Df/esc[2] — it’s okay). No males yet to score phenotype AM collection 2x … Continue reading →

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Sir Paul Nurse seminar

Posted on February 6, 2013 by admin

‘Making Science Work’ good decisions about what research should be supported for the public good. Wilson ‘should not be judged purely in terms of utility’. Wilson quote on how Fermi accelerator contributes to the defense of the nation ‘not the … Continue reading →

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Wednesday 02/06/13

Posted on February 6, 2013 by admin

9:00 A – 8:30P, 9:30P-11:00P Mulitplex STORM covergalss shattered en route from HMS to cambridge, extracting fragments of slides, successfully ID Bx-C Pc. stained BX-C with Pc-488 (new ab-cam anti-rb). Stained additional Pc labeled slide with Pc-750. (1 hr + … Continue reading →

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Tuesday 02/05/13

Posted on February 5, 2013 by admin

9:30 A – 7:30P (lab) 8:30P – 2:40A (remote) Coding troubleshooting issue with Insight’s Z-fits not matching mine. Try flipping wx and wy? try flipping zc = zlevel – zstage Maybe the issue is in the write inifile / which … Continue reading →

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