Monthly Archives: August 2013

Protected: New OligoPaints Protocol

Posted on August 26, 2013 by admin

There is no excerpt because this is a protected post.

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Sunday 08/25/13

Posted on August 25, 2013 by admin

2:45p – 7:15p Review revising introduction of models Cell culture clone8 cells growing fast. A bit overgrown, need to split this afternoon. separated ‘non-adherent’ fraction of Kc167 cells remain primarily non-adherent. Adherent fraction of remains adherent. test plate Kc167 cells … Continue reading →

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Saturday 08/24/13

Posted on August 24, 2013 by admin

4:00p – 6:30p (420 regatta day) Coding Turned splitQVdaxFast into a function. Overwrote splitQVdax original, made that file into splitQVdaxM (for mem-map). overall it looks like memory mapping dax files is a bad idea. They are too big. Running new … Continue reading →

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Friday 08/23/13

Posted on August 23, 2013 by admin

10:15a – 8:10p, 8:50p-11:05p BX-C tiling Finish O/N STORM of Ubx spots start 65C 50% formamide rinse out of probe (30 min) Hot wash of newly labeled Ubx (orginally AbdA): 60C 2x SSCT Image Ubx. Correspondence of cells and correspondence … Continue reading →

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Thursday 08/22/13

Posted on August 22, 2013 by admin

10:10a – 7:00p, 8:50p – 12:10a STORM & staining BX-C tiles AbdA labeled coverslip (S2 cells) found cross-hatching imaging additional cells record chromatic beads will restain AbdA-stained coverslip with BX-C Ubx labeled coverslip (S2 cells) found cross-hatch (much more evident … Continue reading →

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Wednesday 08/21/13

Posted on August 21, 2013 by admin

9:45a – 11:30p cell staining heating buffer. wrong buffer, no formamide in 60C hot wash. Did I use the formamide buffer yesterday? I recall heating a 50mL tube and I don’t think I have 2x SSCT in a 50mL tube… … Continue reading →

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Tuesday 08/20/13

Posted on August 20, 2013 by admin

9:45a – 5:15p, 10:00p-12:25p General stuff mailroom still closed (10a) still can’t pick up package need to get PCR tubes, razor blades and 10x PBS from stock room. cell staining hot wash 65C in waterbath 17 min. no staining (low … Continue reading →

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Monday 08/19/13

Posted on August 19, 2013 by admin

9:30a – 8:30p Banff to do email speakers who haven’t sent in titles (individual emails sent). update schedule on website with tentative speaker order both goals complete for today Probe making oligo cleanup of probes new gel from O/N gel-green … Continue reading →

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Sunday 08/18/13

Posted on August 18, 2013 by admin

12:20p – 7:00p, 8:40p – 11:30p Goals reread and edit review analyze latest round of BXC data Probe making verify direction of MYcroArray BX-C Hox probes: probe region is “+” strand (antisense) T7 is on the nick side primer –> … Continue reading →

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Saturday 08/17/13

Posted on August 17, 2013 by admin

10:30a – 10:30p Helping Hao align FRET Wrote SplitQVdax.m SplitDaxFRET_130817 Note: splitting large dax files is slow and memory intensive. Even 2000 frames takes a few gigs in the memory map approach. (10:30-2:30). Will need to help get setup with … Continue reading →

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