Monthly Archives: August 2015

9:45 am – 8:00 pm, remotely, 9:15 pm – 11:00 pm RNAi validation Finish library prep run final bead purification and elution background prep contact Bauer core to confirm dates for tape analyzer reading background for KAPA_Library_Quantification stresses don’t freeze … Continue reading →

9:45 am RNA library prep setup protocol from NEB discuss plans with Jeff request training on BioAnalyzer and/or TapeStation instrument for library QC collecting kit components RNA purification run out 1 uL on a 1% agarose gel in TAE treat … Continue reading →

11:00 am – 7:45 pm RNAi QPCR Oligo cleanup purified T7 DNA (probably could have done a standard DNA cleanup, I don’t need the supershort 30 bp stuff) ran plate of QPCR running out of primers. Should order more primers … Continue reading →

9:00 am – 5:45 pm Plan need to do fewer review experiments at once so we can do them more carefully should focus on the live imaging and Pc analysis need to get good QPCR need to set up deep-sequencing … Continue reading →

9:30 am – 7:30 pm, 9:00 pm – 11:45 pm Chromatin Project RNAi RT reactions protocol 5 uL gene-specific primer mix 10 uL total RNA, (diluted to 400 ng/uL) 5 uL RT buffer 1 uL dNTPs 1 uL Maxima 2 … Continue reading →

9:45 am – 11:30 pm To do submit review book flights for EMBO (travel form / shuttle requests due on Friday) Chromatin project centromeric heterochromatin downloaded dm3 heterchormatic assemblies from UCSC here: http://hgdownload-test.cse.ucsc.edu/goldenPath/dm3/chromosomes/ mapping previously imaged GREEN regions back to … Continue reading →

10:00 am – 5:15 pm, 8:30 pm – 1:15 am tasks pick up Ph antibody from Patrick at MGH (antibody gift from Jurg Muller) ELYRA training. — with extreme laser power images looked promising. Otherwise images looked substantially less nice … Continue reading →

10:00 am – 11:00 pm No lab meeting STORM Fixed focus lock error with Hazen on STORM2, 10am – 12pm Set up O/N imaging of L2F03F04 started analysis of STORM data Analysis analyzing live imaging data from last night looks … Continue reading →

11:00 am – 1:15 am PcG RNAi New Knockdowns and cell culture plate Ph, Pc, and mock RNAi cells onto coverglass density looks okay modulo a decent number aren’t sticking isolated cells for RNA extraction fixed plated cells to coverglass … Continue reading →