Tag Archives: coding

Tuesday 10/15/13

Posted on October 15, 2013 by admin

11:00am – 12:30p Fly work Flip stocks terminate bottle stocks Coding working on ChromatinCropper added user controls added drift correction options changed display changed export / save data methods STORM STORM of G5-P2-Cy3 with G4-P1-405 Little/no signal in Cy3 channel … Continue reading →

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Monday 10/14/13

Posted on October 14, 2013 by admin

9:30a -12:30p STORM Analysis setting up more dot-finding analysis on Cajal setting up final splitQVdax for current data. Running stv2mat on all Mosaic data not yet processed. F10 seems to have issues running a complete round of splitQVdax. Some movies … Continue reading →

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Friday 10/11/13

Posted on October 11, 2013 by admin

10:00a – 10:30p Goals send Ph modeling results to Ajaz work on presentation send reminder about racing Saturday STORM analysis Copying F11 data to ProBox2. Delete data from Storm2 Running splitdax on no405Primary, E07fail, F09, and F01v2 (latter 2 including … Continue reading →

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Wednesday 10/02/13

Posted on October 2, 2013 by admin

10:00a – 11:30p Goals today repeat plate PCR, work on technique, set up for sequencing STORM pipeline, G10 425kb black. start new small black. work on Banff Report – NO PROGRESS work on slides for XZ meeting – [Full time … Continue reading →

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Wednesday 09/18/13

Posted on September 18, 2013 by admin

9:15a – 11:30p Writing working on BIRS workshop report (9:15a- 12p) Probe making / training with Bogdan test PCR from yesterday common primer concentration was a bit low gel didn’t fully dissolve, might have affected gel running: Repeat PCRs. Repeat … Continue reading →

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Friday 08/30/13

Posted on August 30, 2013 by admin

8:30a-5:00p Goals for today RNA clean up of pilot Lib2 templates. comparison of SuperscriptII and SuperscriptIII RT yields plate scale in vitro transcription? Align multi-day stain get comments on review Coding writing script to aid finding cell matches from Mosaics … Continue reading →

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Thursday 08/29/13

Posted on August 29, 2013 by admin

8:15a – 7:00p Multi-stain analysis try crosslinking 415 beads to fresh, unfixed cells initial beads somewhat clumped and extremely bright, especially under high 405 illumination (or minimal weak 488 illumination). Beads do successfully blead into 647 channel at moderate brightness … Continue reading →

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Saturday 08/24/13

Posted on August 24, 2013 by admin

4:00p – 6:30p (420 regatta day) Coding Turned splitQVdaxFast into a function. Overwrote splitQVdax original, made that file into splitQVdaxM (for mem-map). overall it looks like memory mapping dax files is a bad idea. They are too big. Running new … Continue reading →

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Friday 08/23/13

Posted on August 23, 2013 by admin

10:15a – 8:10p, 8:50p-11:05p BX-C tiling Finish O/N STORM of Ubx spots start 65C 50% formamide rinse out of probe (30 min) Hot wash of newly labeled Ubx (orginally AbdA): 60C 2x SSCT Image Ubx. Correspondence of cells and correspondence … Continue reading →

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Thursday 07/25/13

Posted on July 25, 2013 by admin

STORM break down O/N run of R2bxcD STORM analysis run all bxc data dot-fitting (que up on Tuck in small batches, Cajal not talking) run all bxc mosaic file conversions (que up on Cajal in linear batches) Embryo staining incubating … Continue reading →

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