Tag Archives: fly work

Friday 05/04/12

Posted on May 4, 2012 by admin

12:00 P – 9:30 P 8:30-12P volleyball practice update probe & plasmid library update antibody labels collect virgins set up shRNA crosses x Pc shRNA2 and Psc shRNA 1 in collection cages PCR for in vitro transcription rxn to make … Continue reading →

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Wednesday 05/02/12

Posted on May 2, 2012 by admin

10:25 A – 8:50P Wash out O/N H3K27me3 on Pc-GFP labeling embryos Etch H4 (H3K27, K4): 1h 25 min sequences back from GeneWiz: Fab8B +/-, inside msa ncRNA (-) Fab8A, +/- inside msa ncRNA (-) AbdB-2B +/+ antisense. use T7 … Continue reading →

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Monday 04/23/12

Posted on April 23, 2012 by admin

9:45 A – 11:30P Lab meeting presentation John Wu’s thesis defense Consolidated disk drives: new data on external drives Alistair4 and Alistair5 backed up to empty gaps slots on Alistair2 and 3. Flip fly cages – 2:30P Analyze Psc localization, … Continue reading →

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Sunday 04/22/12

Posted on April 22, 2012 by admin

10:30 A -5:00P Fly work Flipped all Gal4-Bcd3’UTR lines into new bottles (8).  Moved to 25C.  Flip again in 2-3 days, start virgin collection in 10 days. New cages of MP09, yw, and Pc-GFP. Disposed all bottles of MP09 and … Continue reading →

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Sunday 04/01/12

Posted on April 1, 2012 by admin

8:50 A — 11:30P Finish O/N confocal of MP08. For Zeiss710: a 1.7 um offset from center, with 8 um difference between top plane and imaging plane for the first sample only (with other samples all set to top plane) … Continue reading →

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Sunday 03/18/12

Posted on March 18, 2012 by admin

11:00 A – 8:45 P No colonies on plates Finish O/N STORM run.  Run failed at movie 121, unknown error message. attempt bead feducial drift correction: polysterene beads good autofluoresnce in all but 750, but bleach fast. 405 beads not … Continue reading →

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Friday 03/02/12

Posted on March 2, 2012 by admin

10:05 A -1:00 A Flip new PcG shRNA lines. Enter into stocks. Screen MP12 rescue crosses.  set up 6 new crosses, 3 with bright eyes might be homozygous MP12. 1 vial with straight wings tossed and 1 vial with Sp … Continue reading →

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Thursday 03/01/12

Posted on March 1, 2012 by admin

9:50 A – 12:10 A Transfer PcG and Hb/Bcd samples to ethanol for embedding Running Insight3 analysis on single section images from Tuesday / Wednesday STORM session. Discussion with Graham and Colenso: Try switching to BME for Drosophila PcG shRNA … Continue reading →

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Wednesday 02/29/12

Posted on February 29, 2012 by admin

9:45 A — 11:00P Washing out O/N 4C antibodies from new protein stains Flip Fly stocks Flip vials and enter new GFP lines into fly stocks. Start expanding Gal4 bcd-3′ UTR (to try crossing into PcG RNAi lines.  will need … Continue reading →

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Monday 02/20/12

Posted on February 20, 2012 by admin

9:45 A — 10:00 P Transfer embryos into 30% resin Group meeting / BPS practice talks Transfer embryos into 50% resin New collection cages: 4x snail, yw, and MP09s. embryos into 70% resin.  3 hrs, then 100% resin. images uploaded … Continue reading →

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