Tag Archives: images

z-calibration: Insight vs DaoSTORM

Posted on February 13, 2013 by admin

with single iteration, Hazen 3D-DaoSTORM avoids splitting molecules more distant from frame A few notes on 3D-DaoSTORM First 4 iterations are computed with 4x the input threshold. So if using less than 4 iterations you want to quarter the threshold … Continue reading →

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chromatic warping: general formulation

Posted on February 11, 2013 by admin

Description of problem There are N different color channels which are imaged through different filters, and need to be mapped together to an accuracy greater than the resolution limit of the instrumentation / detection system (e.g. 10-20 nm for STORM, … Continue reading →

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BXC + H3K27me3 first try

Posted on January 27, 2013 by admin

  K27me3 post-in situ labeling turned out pretty sparse.  Still have a few thousand localizations for which cluster nicely in several cases at the BX-C locus.   Overall this 488 staining is very sparse, there are maybe an order of … Continue reading →

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Protected: BX-C clusters first look

Posted on January 26, 2013 by admin

There is no excerpt because this is a protected post.

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Thursday 01/24/13

Posted on January 24, 2013 by admin

9:30 A – 7:30P STORM * Finish O/N run by 10A. * Run Z-calibration * Copy and delete data!  Remove external hard-drive * Image BX-C + H3K27me3-488 slide (at 4C in PBT).  maybe we should store these in 2x SSCT? … Continue reading →

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Tuesday 01/22/12

Posted on January 22, 2013 by admin

  Fly Work collect virgins (df/CyO-Esc[2])  AM # New crosses with remaining MTDs! # collect virgins PM Ultra-cryo sectioning yw embryos Cutting sample at -51C, knife at -54C, TG -50C at .65 – .75 um cuts pretty well, still better … Continue reading →

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Thursday 01/17/13

Posted on January 17, 2013 by admin

10:30 A – 7:00P, 8:30P – 9:30P (8:30 – 10:30 workout session) Probe making combine, precipitate and pellet en-647 oligoPAINT probe # Wash, combine, and resupend probe-pellet, # Prep for digestion reaction Cell culture (500 kbp probe test 2) After … Continue reading →

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Sunday 12/02/12

Posted on December 2, 2012 by admin

10:30 A – 7:30P, 8:30P – 11:45P Lab meeting prep collecting dual labeled images to show attempts with flanking probes. PCR Test run gels from Espl screening.  Gel 1 from BioXAct (3A and 4A, see legend in yesterday’s post), numerous … Continue reading →

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Friday 11/30/12

Posted on November 30, 2012 by admin

9:30A – 10:15 P Fly Work Collect sna[18] x Double balancer virgin and male F1s PM virgin collection STORM2 time. Colenso removing gas laser from STORM2 Mount ultra cryo section embryo for imaging sections not flat or hard to find. … Continue reading →

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Sunday 11/18/12

Posted on November 18, 2012 by admin

10:15 A – 6:50 P, 7:50P – 10:30P Probe making have not confirmed nicking enzyme concentration for digestion.  Canceled PAGE introduction with Bryan, will attempt again later. Telomeres Read over telomere manuscript from John Wu  Imaging telomeres: 656 = 60 … Continue reading →

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