Tag Archives: embryo labeling

Monday 07/30/12

Posted on July 30, 2012 by admin

9:50 A – 5:00 P, 9:30P – 10:45p Jiang lab meeting difficulty connecting to notebook 710 confocal all sessions canceled, new 780 being installed this week.  Requested training on ELYRA which also has a 710 (and maybe a programable stage?) … Continue reading →

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Tuesday 06/26/12

Posted on June 26, 2012 by admin

10:30 A -5:00P, 9:00P – 11:30P in situs day 2 short fix 2-14 hour yw and Pc embryos. rehydrate and stain 2-14 hour short fix embryos with Su(Z)12.  Pc-GFP embryos also stained with ch a-GFP. vball lost. Synthesized Bac 1 … Continue reading →

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Saturday 05/05/12

Posted on May 5, 2012 by admin

11:00A -5:30P Talk title for Poland wash out primary antibodies Nanodrop gel-extraction samples STORM cryo-section samples: considerable out of focus light makes for hazy looking conventional images, though nuclei and membranes are clearly distinguishable in the respective channels. 647 STORM … Continue reading →

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Friday 05/04/12

Posted on May 4, 2012 by admin

12:00 P – 9:30 P 8:30-12P volleyball practice update probe & plasmid library update antibody labels collect virgins set up shRNA crosses x Pc shRNA2 and Psc shRNA 1 in collection cages PCR for in vitro transcription rxn to make … Continue reading →

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Wednesday 05/02/12

Posted on May 2, 2012 by admin

10:25 A – 8:50P Wash out O/N H3K27me3 on Pc-GFP labeling embryos Etch H4 (H3K27, K4): 1h 25 min sequences back from GeneWiz: Fab8B +/-, inside msa ncRNA (-) Fab8A, +/- inside msa ncRNA (-) AbdB-2B +/+ antisense. use T7 … Continue reading →

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Thursday 05/26/12

Posted on April 26, 2012 by admin

10:00 A – 11:45 P Check colonies from Bx-C round 1 cloning: all plates look good, no lawns. Gel-extract linearized Taf, Tub Gel-extract newly cloned Bx-C probes Antibody synthesis: antibodies 61-69 Flip collection cages: 10:15 A Washing out primary antibody … Continue reading →

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Wednesday 04/25/12

Posted on April 25, 2012 by admin

9:45 A – 8:30 P O/N hybridization — should do in PCR block.  Water evaporates from 37C block, not good contact with PCR tube.  Refilled water, allow another hour of hybrdization before washes. Sequencing results:  A1 = Taf1 antisense (sequence with m13F … Continue reading →

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Thursday 04/12/12

Posted on April 12, 2012 by admin

9:20 A – 11:50 P Both large and small plates look good. large plates a bit sparse but still good colonies to chose from. Benny Shilo seminar coding automated iterations of z-fitting in matlab. oligo-paint- good staining!  high DNA background … Continue reading →

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Thursday 04/05/12

Posted on April 5, 2012 by admin

10:00 A — 8:30 P Take GFP embryos out of 4C, incubating at RT heating up 2x SSCT for hot wash mounted oligo-paint embryos before and after washes (with WGA-488 for post-wash embryos).  Remaining embryos at 4C Cut sections of … Continue reading →

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Wednesday 04/04/12

Posted on April 4, 2012 by admin

9:35 A – 7:30, 9:30 – 10:50 P wash out O/N antibodies Flip fly collection plates 10:10A Request more time to write review Flip expanding Gal4-Bcd3’UTR lines:  growing slowly only 2 bottles worth O/N collection plates of en-GFP, Bcd-GFP, Pc-GFP … Continue reading →

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