Monthly Archives: April 2011

Probe template making

Posted on April 19, 2011 by admin

b-full, sog (R30), sna (R16), y(i), y(f), tupA, pnrA, thsA Most lanes too much loading DNA (amplification efficiency looks inversely proportional to the amount of DNA). Gel extract: snail is too low: 3 ng/uL, others are 14-70 ng/uL.     … Continue reading →

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Monday 04/18/11

Posted on April 18, 2011 by admin

10:15 A – 7:00P, 8:00P – 9:30P Restart MP01_22C_f analysis code, (memory error at emb 8). Flip collection cages MP08 sna[D], 11:00A. Thesis writing. Sequencing results back:  tupA +/+ = SP6, tupB random 3R DNA, thsA +/- = SP6, thsB … Continue reading →

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Friday 04/15/11

Posted on April 15, 2011 by admin

10:00A — 8:45 P Flip collection cages, 10:30A Received fall course evals for BioE190 & 290 Running dot-counting analysis on MP02 data writing README for Ecology paper supporting code. Flip early-rescued hb control BAC flies. Water Perry’s flies. miniprep ths … Continue reading →

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probe competition control

Posted on April 14, 2011 by admin

shorter probe worked (700 bp, yF1 to yR1, terminal part of yellow) longer probe failed (should be 800 bp).

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Thursday 04/14/11

Posted on April 14, 2011 by admin

10:15 A – 5:15 P, 11:00 P -12:30 A Flip collection cages: MP06, MP01, MP02, MP10; sna[18], MP08; sna[D], 10:15A.  Freeze MP09 Hz. Flip collection cages MP06, MP01, MP20, MP10; sna[18], 12:15 P. Check plates: colonies look good for all … Continue reading →

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Wednesday 04/13/11

Posted on April 13, 2011 by admin

9:00 A -5:30P, 8:00P – 11:00P Meeting with Justin, discuss grad schools Gel test PCRs for pnr tup and ths exonic probes discuss snail data with Jacques – combine with Joe’s analysis? flip collection cages, MP01, MP06, MP02, MP09, MP10; … Continue reading →

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more imaging of bursting

Posted on April 13, 2011 by admin

cadN images:  

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new exonic probes

Posted on April 13, 2011 by admin

PCR cloning for tup-exon thus-exon and pnr-exon probes (respectively): Gel extracting and ligating: tup 30ng/uL, pnr 15 ng/uL, ths 15 ng/uL Ligate at 10 uL pnr and ths and 5uL tup to 2 uL pGEM, 20 uL rxn.  3 uL … Continue reading →

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updated Q-bio 2011 abstract (submitted)

Posted on April 12, 2011 by admin

Short Abstract — It has been shown that strong gene induction in eukaryotic cells leads to large bursts of gene expression – increasing total mRNA synthesis at the cost of substantially elevating cell-cell variation in expression levels.  We investigate how … Continue reading →

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progress report

Posted on April 12, 2011 by admin

Findings: (1) looping is frequently rate limiting for transcription (see manuscript draft). (2) Bcd activates transcription by affecting promoter priming, not pol II recruitment (unlike most activators from yeast). (3) hb proximal enhancer required for normal levels of total mRNA … Continue reading →

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