Monthly Archives: October 2011

Friday 10/21/11

Posted on October 21, 2011 by admin

10:00 A – 7:00P Sub and coat slides for sectioning later today.  how long should these dry? remember to let gelatin solution cool before adding dissolved chromium solution. lay-out figures in draft text of snail manuscript.  Read and revise for … Continue reading →

Posted in Summaries | Comments Off on Friday 10/21/11

Thursday 10/20/11

Posted on October 20, 2011 by admin

10:00 A – 11:30 P Working on GPU analysis (see post) test PCR of en BAC working on manuscript figure 7: documenting ‘domino effect’ in MP08 embryos. — should really stain and image more embryos. learning Insight3 with Yari — … Continue reading →

Posted in Uncategorized | Comments Off on Thursday 10/20/11

Wednesday 10/19/11

Posted on October 19, 2011 by admin

9:00 A – 6:50P, 10:10P – 11:30P Intro to STORM 2 imaging with Colenso and Yari no nuclei on sample.  🙁 Imaging en inv locus on Zeiss 700, tou-flu and en bio in yw 4 look pretty good.  One good … Continue reading →

Posted in Summaries | Comments Off on Wednesday 10/19/11

antibody labeling details

Posted on October 18, 2011 by admin

first try 647 antibody 280: .522 A405: .205 A647: .1485 second try 647 A647 =.334 + .25 offset A405=-.0768 + .25 offset antibody 280 = .4522 + .25 offset first try 750 D750 = .4889 A405 = .207 antibody = … Continue reading →

Posted in probe and plasmid building | Comments Off on antibody labeling details

complete fluid exchange embryo rinse chambers

Posted on October 18, 2011 by admin

New method for rapid and complete fluid exchange during embryo washes Cut a P1000 tip to fit snuggly inside a 1.5 mL eppendorf with the lid closed. Cut a small whole in the bottom by removing an edge off the … Continue reading →

Posted in Research Planning | Comments Off on complete fluid exchange embryo rinse chambers

Tuesday 10/18/11

Posted on October 18, 2011 by admin

8:30 A — 9:10 P Meeting with Bernhard.  Probably need to cutaway samples — try 2 micron sections with trimming knife. Review dye making with Colenso plasticize remaining samples in cylindrical mounts nicked translation round 2, try dilution series of … Continue reading →

Posted in Summaries | Comments Off on Tuesday 10/18/11

Monday 10/17/11

Posted on October 17, 2011 by admin

9:45 A – 6:30P, 8:00P-9:00P pGEM kit arrived. en primers arrived.  Fisher 50mm petri dishes discontinued, check with Jacques/Dave — need 35 mm lids Upgrading to Linux 11.10 from 11.04. Reschedule meeting with Bernhard for 8:30A tomorrow. Spec and send … Continue reading →

Posted in Summaries | Comments Off on Monday 10/17/11

Friday 10/14/11

Posted on October 14, 2011 by admin

11:00 A — 9:30P Trying Prep 16A for BAC DNA.  Isopropanol amounts seem off for volume used, increased to 1.75 mL (50%). Prep works fine, conc 380-580 ng/uL.  Prepping second half to increase total DNA nano-section with Yari (3p – … Continue reading →

Posted in Summaries | Comments Off on Friday 10/14/11

Thursday 10/13/11

Posted on October 13, 2011 by admin

10:15 A – 6:30 P Confocal not all embedded samples same color: probably incomplete resin mixing removal of ethanol? not look good. Sample labels: 1-3 LamS, 4-6 BcdA, 7-9, BcdS, 10-12 LamA New Pacman BAC prep protocol cleaner but even … Continue reading →

Posted in Summaries | Comments Off on Thursday 10/13/11

Wednesday 10/12/11

Posted on October 12, 2011 by admin

10:40 A -6:30, 8:00P – 8:45P continue resin embedding washes.  Need light centrifuge to sediment embryos by 1:1 ethanol:resin mini-prep BACs grown O/N (16 hrs) in LB-chlor. media.  All have very low dirty yield (8-30ng/uL) probably not BAC DNA.  BACs … Continue reading →

Posted in Summaries | Comments Off on Wednesday 10/12/11