Monthly Archives: January 2012

Monday 01/23/12

Posted on January 23, 2012 by admin

9:45 A – 9:30P Lab meeting (Guisheng) 10-1p working on fellowship app. Francis lab journal club 4p -6p New antibodies from Francis lab: anti-PscB (affinity purified), anti-Pc, anti-Pho, anti-dsfMBT, anti-Su(z)12 reorganized draft written.  need to review. fellowship to do: reorganize … Continue reading →

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Sunday 01/22/12

Posted on January 22, 2012 by admin

6:00 P – 10:00 P Updating antibody concentration calculations using correct Cy3B details from JV GD and JV have different stats for Cy5. low concentrations make labeling ratios inaccurate. Ordered protein concentrating vials (vivaspin 500). Ordered more Dm0 ADL84 to … Continue reading →

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Saturday 01/21/12

Posted on January 21, 2012 by admin

8:45 A — 2:00 P in situs moved through resin, dense collection of embryos 10 embedding capsules of embryos made for each of the 4 in situ tubes (+4 of tube 1 by KE for practice). in situ embryos baking … Continue reading →

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Friday 01/20/12

Posted on January 20, 2012 by admin

10:10 A -11:00 P labeling primary anti-histone antibodies (see Alistair_STORM_dyes log): pan-H3, H3K4me3, H3K27me3, and H3K27ac.  Need to filter Ac and K27 through column before binding.  Also filtered H3 and K4 — dilutes sample sufficiently to allow for multiple subsequent … Continue reading →

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3D histone views

Posted on January 20, 2012 by admin

3D reassembly of microsection stack of STORM images of individual nuclei with pan-histone marker.  Some alignment issues still, but worth a comparison

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Thursday 01-19-11

Posted on January 19, 2012 by admin

10:30A -8:00P in situs day 3 with KE working on snail paper figures STORM labeling antibodies: see STORM_dyes. attempted direct labeling of primary H3K27me3 with 750 and H3K27ac with 647.  essentially no dye sticks.  Try to repeat with diluted antibodies … Continue reading →

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Wednesday 01-18-12

Posted on January 17, 2012 by admin

10:30A – 8:30 P Make new large fly-plates. Flip collection cages, 11:45 A in situs day 2 with KE (started 11:30A) Flip collection cages 3:30 P (pretty weak lay for 4hrs two big cages) Fix embryos 4:45 P 1-5 hr … Continue reading →

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Tuesday 01-17-12

Posted on January 17, 2012 by admin

9:30A – 7:00 P in situs day 1 + training KE Flip Fly cages 10:45 A Flip collection cages: 12:45 P 3:45PM fix embryos (2-5hr MP08 collection) working on figure for fellowship app

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Monday 01-16-12

Posted on January 16, 2012 by admin

6:30P – 11:00P working on figures for fellowship apps images of open and closed engrailed loci confocal, engrailed slides 6-20-11.  imaging slide 4 ON. comparing probe labeling efficiencies:  tou-dnp does not work, tou-flu weak and backgroundy.  E(Pc)-dig is strong, en-bio and … Continue reading →

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STORM imaging progress update

Posted on January 14, 2012 by admin

Review of recent progress on multicolor STORM imaging of gene regulation. WGA labeling   membrane in 100 nm section.  little Hb detected (red stain mostly background). en-dig stains well with H3K27.  just need to cut it better. AbdA (red) and Ubx (green) … Continue reading →

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