Monthly Archives: February 2012

Friday 02/09/12

Posted on February 10, 2012 by admin

10:10 A — 11:20 P move samples from 4C, change 100% EtOH STORM: setup 10x objective for finding samples. substantial offset, need to calibrate. Need to set laser powers to zero but have lasers on before starting dave! Ordered Bloomington … Continue reading →

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whole-mount tests 02/09-10/12

Posted on February 10, 2012 by admin

slide H1: 4 color anti-nuclear lamin test   slide H2: Directly labeled antibodies: Dm01-488(green) H3K27me3-Cy3B (red), H3-Cy5 (blue), H3K27ac-750 (not shown) slide H4: H3K27me3-Cy3B (red) and H3K4me3-Cy5 (blue) along with 405 background (green). slide H4: earlier embryo: H3K27me3 ‘clusters?’  (red) … Continue reading →

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Thursday 02/09/12

Posted on February 9, 2012 by admin

9:50 A – 1:00A Remove and keep primaries from all staining reactions (except traditional snail labeling of MP08 embryos) Washing embryos, in situs / staining reactions day 3. new stock of 1x PBT clean frozen fly cages Flip fly cage: … Continue reading →

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Wednesday 02/08/12

Posted on February 8, 2012 by admin

10:00 A – 8:45P in situs day 2 (no KE) antibody test try 2: 15 min fix <24 hr methanol treated embryos stained for PcG proteins. much higher concentration of histone primary antibodies test stain (record) Trial run of new … Continue reading →

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Tuesday 02/07/12

Posted on February 7, 2012 by admin

9:45 A — 8:00 P screwed up over-night run 561 channel for Ubx data — set delay to 15,000 and scan time 200 rather than scan to 15,000 frames. Analyzing STORM data from Sunday and Monday. batchinsightM not working right. … Continue reading →

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Monday 02/06/2012

Posted on February 6, 2012 by admin

9:45 A – 1:30A Flip fly plates, 10A lab meeting — planning STORM workshop. Fix embryos, 15 min wt fix, 2-5 hrs. 25 min MP08 fix, embryos added to todays in situs. STORM analysis to test parameters for S45 data. … Continue reading →

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Sunday 02/05/12

Posted on February 5, 2012 by admin

9:40 A – 8:30P, 10:00P – 1:00A Flip fly plates try relabeling Dm0 antibodies with cy3B and 647 (first round labels still pretty concentrated protein.  Most of this is not antibody but we just reduce the dilution when adding to … Continue reading →

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Saturday 02/04/12

Posted on February 4, 2012 by admin

11:15 A – 7:30P Flip collection cages, 11:20 A Write JTB review Aliquot new dyes label new laminin Dm0 antibodies.  Protein conc very high, low labeling frequency.  see STORM antibody record. imaging failed.  had focus lock on wrong objective for … Continue reading →

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Friday 02/03/12

Posted on February 3, 2012 by admin

10:10 A – 9:30 P Flip collection cages, 10:15 A Prep new coverslips (2 racks) Flip collection cages 12:15 P clear sna[D] flies for virgin collection Flip fly stocks Flip MP09 and 4x snail flies transfer laid embryos to make … Continue reading →

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Thursday 02/02/12

Posted on February 2, 2012 by admin

9:30 A – 7:00P, 8:45 – 12:30A List of contacts to write to for BIRS, sent to co-organizers Flip fly cages 10:15 A.  Keep plates. Turn on humidifiers for sectioning room, 10:30A Check baked resin in chamber slides on confocal. … Continue reading →

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