Monthly Archives: July 2012

Monday 07/23/12

Posted on July 23, 2012 by admin

5:00P – 12:00 A Recomputing polyermase density for snail looking for twinned spots. Reasonably common. Half of cc13 embryo collection imaged is probably clean through replication of sna locus. No G phases in early division (O’Farell 1994).  cc14 is the … Continue reading →

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SDB 2012 notes

Posted on July 23, 2012 by admin

Live transcribed / unedited notes: Notes: Nicole King (Coenos) Many cell-cell interaction genes present in Coenos. ‘evo-cell’ how did specialized animal cell first evolve? Role of bacteria in shaping evolution of development Earh 4.6Ga, first life ~3.6Ga, Multicell 1.0Ga – … Continue reading →

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Wednesday 07/18/12

Posted on July 18, 2012 by admin

10:10A – 9:30P working on new SDB talk (kinetics, no bistability) Issue with text animation.  Just on windows system. working on bead fitting.  06-25-beads (100nm) fit pretty well, see all 5 regions in most channels (vis have 4 clear and … Continue reading →

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Tuesday 07/17/12

Posted on July 17, 2012 by admin

10:10 A – 7:00P, 8:20 P – 11:10P working on presentation for SDB teaching primer design for KE: new primers for: bxd, lab, #scr, CG5520, CG3995, CG10326, CG10324.  Ordered. KE confocal of last weeks embryo staining in vitro transcription reactions … Continue reading →

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Monday 07/16/12

Posted on July 16, 2012 by admin

9:40A – 5:00P Etching s6: 5E-dig, AbdB-bio.  K27-647 looks good on Turnkey.  Etched 22 min in second use of sodium ethoxide (at 22C for 2 weeks, now dark brown). Review knife care with KE.  (Trimming knife was badly chipped during … Continue reading →

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Friday 07/13/12

Posted on July 13, 2012 by admin

9:50 A – 7:00P Submit kinetics paper John recommends 3 into 20 dilution of BAC probe, stain on slide in a sandwich between coverglass and a slide.  Use heat block with insert upside down for hybrdization clean up Snail Doc … Continue reading →

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Thursday 07/12/12

Posted on July 12, 2012 by admin

10:15 A — 8:20 P, 9:20P – 12:00A Finish writing draft cover letter for kinetics paper.  Send to ML. in situs embedding (day 4) KE. coat new coverslips KE. update code repos (need to do this more often). working on … Continue reading →

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Wednesday 07/11/12

Posted on July 12, 2012 by admin

10:10 A – 5:ooP check antibody locations with KE in situs day 3 Confocal: tub and Ant-C genes look good.  Preliminary data suggests loci mostly paired in older embryos but not condensed — substantial separation distance. Ubx and AbdB have … Continue reading →

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Tuesday 07/10/12

Posted on July 10, 2012 by admin

10:10 A – 5:15 P, 10:30P – 12:45A etching H3K4 4-color coverslip 3 in new sodium ethoxide (pale yellow color) for 13 min = mostly etched, resin definetely in the doubled over sections, some patches on single embryos.  clear bur … Continue reading →

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Monday 07/09/12

Posted on July 9, 2012 by admin

9:50 A – 6:50 P remove slide 3 of H3K4-section from 60C heat block, add to slides. Hazen Lab meeting 3D viewing in Insight:  ctrl+shift+x, select ‘no’, ctrl+x gives 3D viewer.  Need to have color shading as percentage toggled on … Continue reading →

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