Monthly Archives: February 2013

Protected: lab meeting 02/18/13

Posted on February 18, 2013 by admin

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Sunday 02/17/13

Posted on February 17, 2013 by admin

STORM Finish O/N run of BXC + flank try2 (3D this time). Did not lose TIRF. Did not overfill drive or crash computer (4GB left on hard-drive, 400 written). Copying to new Alistair10. (will move to the new Alistair9 when … Continue reading →

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Saturday 02/16/13

Posted on February 16, 2013 by admin

10:30 A – 8:30P, 10:00P – 11:55P STORM Analysis DaoSTORM analysis still running on Fab7-A647 Pc-750 Read write steps from USB connected hard-drive very slow when this analysis is running. (I’m not sure how much slower the analysis goes to … Continue reading →

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Friday 02/15/13

Posted on February 15, 2013 by admin

9:30 A STORM Finish O/N run crashed hal when computer memory filled up. Need more external harddisks! Should do a test run on if we can save data on the fly from Hal over the network straight to Monet. Still … Continue reading →

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Thursday 02/14/13

Posted on February 14, 2013 by admin

9:20 A -7:00P, 9:00P – 10:30P STORM Finish O/N confocal More cells not in TIRF – sections not bleaching, even 647. Dropped TIRF angle to 14.2, now switching well, still good contrast. Might need to re-image this slide later today. … Continue reading →

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Wednesday 02/13/13

Posted on February 13, 2013 by admin

9:50 A — 7:00P, 8:00P – 11:15P Fly Work flip collection cage, 9:50 A Collect virgin Mat-alpha-tubs, esc/CyO x da[10]/Gla and esc/esc mutants. two new bottles of mat-alpha-tubs cleared and added to virgin collection 1 hatched larvae in Mat-alpha-tub x … Continue reading →

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3D chromatic warp: Insight vs DaoSTORM

Posted on February 13, 2013 by admin

Results using 2013-13-02 Bead Data: Z-calibrations for this data set look pretty similar Comparing Chromatic warping DaoSTORM is actually getting a better fit and tighter warp, but not managing to get the same dynamic range in Z — only +/- … Continue reading →

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z-calibration: Insight vs DaoSTORM

Posted on February 13, 2013 by admin

with single iteration, Hazen 3D-DaoSTORM avoids splitting molecules more distant from frame A few notes on 3D-DaoSTORM First 4 iterations are computed with 4x the input threshold. So if using less than 4 iterations you want to quarter the threshold … Continue reading →

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Tuesday 02/12/13

Posted on February 12, 2013 by admin

9:30 A – 7:00P, 8:45P – 11:45P (remote) Coding changed way z-drift is calculated during z-calibration movie: assume that first jump is the intended distance of 600 nm (well we actually compute this back by taking max(zstage – zstage(1)) *1000 … Continue reading →

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Monday 02/11/13

Posted on February 11, 2013 by admin

9:30 A – 7:15, 10:00P – 11:45P Postdoc candidate see lab meeting notes Fly Work Flip collection cage 9:30A. O/N collection poor, not worth fixing. good collection at 1P, should probably flip around 1:30 and fix later. Collect AM virgins … Continue reading →

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